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KMID : 0043320120350010009
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Archives of Pharmacal Research
2012 Volume.35 No. 1 p.9 ~ p.17
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Bioactivity-guided fractionation and analysis of compounds with anti-influenza virus activity from Gardenia jasminoides ellis
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Yang Quanjun
Wu Bin
Shi Yujing
Du Xiaowei
Fan Mingsong
Sun Zhaolin
Cui Xiaolan
Huang Chenggang
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Abstract
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Bioassay-guided fractionation of extracts from Fructus Gardeniae led to analysis of its bioactive natural products. After infection by influenza virus strain A/FM/1/47-MA in vivo, antiviral activity of the extracts were investigated. The target fraction was orally administered to rats and blood was collected. High-performance liquid chromatography coupled with photo diode array detector and electrospray ion trap multiple-stage tandem mass spectrometry was applied to screen the compounds absorbed into the blood. A structural characterization based on the retention time, ultraviolet spectra, parent ions and fragmentation ions was performed. Thirteen compounds were confirmed or tentatively identified. This provides an accurate profile of the composition of bioactive compounds responsible for the anti-influenza properties.
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KEYWORD
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Bioassay-guided fractionation, HPLC-PDA-ESI-MS/MS, Fragmentation behavior, Anti-influenza
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